Premium
Enhanced function of chondrocytes in a chitosan‐based hydrogel to regenerate cartilage tissues by accelerating degradability of the hydrogel via a hydrolysable crosslinker
Author(s) -
Ishikawa Shohei,
Iijima Kazutoshi,
Matsukuma Daisuke,
Iijima Michihiro,
Osawa Shigehito,
Otsuka Hidenori
Publication year - 2020
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.48893
Subject(s) - glycosaminoglycan , cartilage , self healing hydrogels , extracellular matrix , chemistry , hyaline cartilage , chondrogenesis , regeneration (biology) , chitosan , matrix (chemical analysis) , type ii collagen , tissue engineering , microbiology and biotechnology , biophysics , anatomy , biomedical engineering , articular cartilage , biochemistry , polymer chemistry , osteoarthritis , pathology , biology , medicine , alternative medicine , chromatography
Chitosan‐based hydrogels as scaffolds for culturing chondrocytes were prepared using linkers with and without hydrolysable poly( dl ‐lactide) (PLA) segments. The evaluation of the cultured chondrocytes in them indicated that the accelerated degradation of the hydrogel via hydrolysis of the PLA slightly promoted production of the sulfated glycosaminoglycan and drastically improved that of collagen from the encapsulated chondrocytes, which are the chondrospecific extracellular matrix components. Furthermore, the accelerated degradability significantly upregulated the gene expression for Collagen II production and downregulated that for Collagen I production of the encapsulated chondrocytes. Because major component of the articular cartilage tissue is Collagen II‐rich hyaline cartilage, these results suggest the degradation of the scaffolds is an important parameter in cartilage tissue regeneration and the accelerated degradability may have benefits on promotion of cartilage tissue regeneration especially from the viewpoint of hyaline cartilage‐like collagen production. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 137 , 48893.