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Organic–inorganic collagen/iota‐carrageenan/hydroxyapatite hybrid membranes are bioactive materials for bone regeneration
Author(s) -
Nogueira Lucas F. B.,
Maniglia Bianca C.,
Blácido Delia R. T.,
Ramos Ana P.
Publication year - 2019
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.48004
Subject(s) - membrane , chemical engineering , apatite , contact angle , simulated body fluid , self healing hydrogels , wetting , materials science , aqueous solution , chemistry , precipitation , dissolution , polymer chemistry , organic chemistry , biochemistry , physics , meteorology , engineering
This study aimed to produce biomembranes with controlled degradability for application in bone regeneration in order to stimulate biological reactions necessary to improve bone formation. Hydrogels were prepared by dissolving hydrolyzed collagen (HC) and iota‐carrageenan (ι‐Carr) in aqueous mixtures containing CaCl 2 and H 3 PO 4 . A rise in pH by exposure to NH 3(g) caused mineral precipitation into the hydrogel. Subsequently, the membranes were fabricated by solvent casting. Infrared spectroscopy and X‐ray diffraction attested hydroxyapatite formation. The crystallite size was close to 12 nm, which was smaller than the size reported for human bone apatite. The membranes induced bone‐like apatite precipitation in simulated body fluid. The carrageenan content modulated the membrane mechanical behavior. Membranes with controlled degradability were obtained by using higher amount of this polysaccharide. These membranes were able to release HC in physiological conditions. The surface properties were evaluated in terms of wettability and surface energy (γ S ) by means of contact angle (θ c ) measurements. Low θ c (8.5–16.8) indicated that the hybrid membranes were hydrophilic, while higher γ S values, around 70.6 mJ.m −2 , could favor biomolecule incorporation into the surface. Our data set evidenced that these materials could potentially be used as a temporary guided tissue regeneration membrane with the possibility of inducing bone regeneration. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 48004.