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Effect of different initiators on temperature‐sensitive monolithic columns and application in online enrichment of β‐sitosterol
Author(s) -
Guo Bin,
Wang Huimin,
Lan Danan,
Pang Xiaomin,
Li Yiming,
Liu Haiyan,
Yan Hongyuan,
Bai Ligai
Publication year - 2019
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.47683
Subject(s) - monolith , thermogravimetric analysis , sorbent , adsorption , detection limit , chromatography , polymerization , materials science , solid phase extraction , extraction (chemistry) , chemistry , nuclear chemistry , analytical chemistry (journal) , chemical engineering , catalysis , polymer , organic chemistry , composite material , engineering
Three temperature‐sensitive monolithic columns were prepared by atom transfer radical polymerization and oxidation–reduction method using the initiator systems of CCl 4 /FeCl 2 , BPO/DMA, and APS/TEMED, respectively. The three monoliths were characterized by scanning electron microscopy, nitrogen adsorption, and thermogravimetric analysis. The obtained monolithic columns were used as on‐line solid phase extraction sorbent coupled with high‐performance liquid chromatography for the enrichment of β‐sitosterol. By comparing some influencing factors on the adsorption, the optimum temperature‐sensitive monolithic column which was initiated by CCl 4 /FeCl 2 was selected for enrichment of β‐sitosterol from plant oil. The maximum adsorption capacity of the monolith for β‐sitosterol was 10.0031 mg/g. The limit of detection and limit of quantitation were 0.039 and 0.13 mg/mL, respectively. The recovery was in the range of 90.21–98.26%. The results showed that the monolith had better selectivity for β‐sitosterol and could be used for enrichment of β‐sitosterol in food samples. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 47683.