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Molar mass distribution by size exclusion chromatography: Comparison of multi‐angle light scattering and universal calibration
Author(s) -
Podzimek Stepan
Publication year - 2019
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.47561
Subject(s) - molar mass , size exclusion chromatography , molar mass distribution , polymer , viscometer , gel permeation chromatography , intrinsic viscosity , light scattering , calibration , analytical chemistry (journal) , materials science , multiangle light scattering , refractometry , scattering , chemistry , chromatography , viscosity , optics , organic chemistry , refractive index , mathematics , physics , statistics , optoelectronics , composite material , enzyme
ABSTRACT Series of polymers of various molar mass, chemical composition, and molecular architecture was analyzed by size exclusion chromatography (SEC) coupled with a multi‐angle light scattering (MALS) photometer and an online viscometer. The molar mass averages were determined from the signal of MALS or calculated from the intrinsic viscosity and universal calibration. The comparison of the obtained results showed significant differences between the two methods. The MALS detection was shown to be more accurate for the determination of the weight‐average molar mass and less vulnerable to the spreading of polymer peak by band broadening. The universal calibration can yield more accurate estimation of the number‐average molar mass of branched polymers. It is also significantly more accurate for the characterization of fluorescent polymers than MALS with a regular laser of 660 nm. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 47561.