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Bioinspired glycosaminoglycan hydrogels via click chemistry for 3D dynamic cell encapsulation
Author(s) -
Kuang Liangju,
Damayanti Nur P.,
Jiang Chunhui,
Fei Xing,
Liu Wenjie,
Narayanan Naagarajan,
Irudayaraj Joseph,
Campanella Osvaldo,
Deng Meng
Publication year - 2019
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.47212
Subject(s) - self healing hydrogels , cell encapsulation , 3d cell culture , chemistry , hyaluronic acid , encapsulation (networking) , biophysics , chondroitin sulfate , polyethylene glycol , cell adhesion , tissue engineering , mesenchymal stem cell , glycosaminoglycan , cell , nanotechnology , materials science , polymer chemistry , biochemistry , microbiology and biotechnology , biomedical engineering , medicine , computer network , genetics , biology , computer science
Cell encapsulation within 3D hydrogels is an attractive approach to develop effective cell‐based therapies. However, little is known about how cells respond to the dynamic microenvironment resulting from hydrogel gelation‐based cell encapsulation. Here, a tunable biomimetic hydrogel system that possesses alterable gelation kinetics and biologically relevant matrix stiffness is developed to study 3D dynamic cellular responses during encapsulation. Hydrogels are synthesized by crosslinking thiolated hyaluronic acid and thiolated chondroitin sulfate with poly(ethylene glycol) diacrylate under cell‐compatible conditions. Hydrogel properties are tailored by altering thiol substitution degrees of glycosaminoglycans or molecular weights of crosslinkers. Encapsulation of human mesenchymal stem cells through hydrogel gelation reveals high cell viability as well as a three‐stage gelation‐dependent cellular response in real‐time focal adhesion kinase (FAK) phosphorylation in live single cells. Furthermore, stiffer hydrogels result in higher equilibrium FAK activity and enhanced actin protrusions. Our results demonstrate the promise of hydrogel‐mediated cellular responses during cell encapsulation. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 47212.