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Electrospun‐electrosprayed hydroxyapatite nanostructured composites for bone tissue regeneration
Author(s) -
Gandhimathi Chinnasamy,
Venugopal Jayarama Reddy,
Ramakrishna Seeram,
Srinivasan Dinesh Kumar
Publication year - 2018
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.46756
Subject(s) - biocomposite , electrospinning , fibroin , materials science , osteocalcin , alkaline phosphatase , fourier transform infrared spectroscopy , nanofiber , chemical engineering , ultimate tensile strength , composite material , chemistry , silk , composite number , polymer , biochemistry , enzyme , engineering
Electrospinning and electrospraying process provides a versatile approach for the fabrication of biocomposite nanofibrous scaffolds (NFS) with structural, mechanical, and biological properties is to mimick the native extracellular matrix. The present study was aimed to fabricate NFS of poly( l ‐lactic acid)‐co‐poly(3‐caprolactone) (PLACL), silk fibroin (SF) and hydroxyapatite (HA) by blending (b) and spraying (s) methods. The biocomposite NFS were characterized by field emission scanning electron microscopy (FESEM), transmission electron microscopy, water contact angle, tensile strength, fourier transform infrared spectroscopy (FTIR) analysis. The fabricated biocomposite NFS showed pore size around 1.5–7.8 μm and porosity of 68–93% with fiber diameter ranging from 213 ± 78 to 340 ± 52 nm. Furthermore, the mesenchymal stem cells (MSCs) were cultured on the biocomposite NFS and the cell interaction, proliferation, mineralization, and osteocalcin expression were analyzed by FESEM, MTS assay, alizarin Red S staining, and immunofluorescence analysis, respectively. The presence of bioactive SF/HA molecules supported biological functions namely cell attachment, proliferation, and differentiation of MSCs into osteoblasts. The observed results suggested that the biocomposite PLACL/SF/HA(s) NFS stimulated osteogenic differentiation of MSCs as evident from alkaline phosphatase activity, mineralization, and expression of osteocalcin for bone tissue engineering. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 46756.