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Morphological characteristics of polymeric nylon‐6 film as biological recognition interface for electrochemical immunosensor application
Author(s) -
Shaimi R.,
Low S. C.
Publication year - 2018
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.46741
Subject(s) - polyaniline , glutaraldehyde , materials science , nylon 6 , electrochemistry , reagent , conductive polymer , polymer , bovine serum albumin , analyte , chemical engineering , biosensor , polymer chemistry , electrode , chromatography , composite material , nanotechnology , chemistry , organic chemistry , engineering , polymerization
ABSTRACT The present study elucidates the morphology characteristic of nylon‐6 as protein immobilization film and their influences in biological recognition interface for a single‐use electrochemical immunosensor. A basic requirement of this polymer platform is to allow the testing analyte [conductive polyaniline‐Fe 2 O 3 ‐glutaraldehyde‐conjugated‐ N ‐hydroxysuccinimidobiotin (PANI‐Fe 2 O 3 ‐GA‐b‐NHS)] to access to the immobilized capture reagent [bovine serum albumin (BSA)] on a surface of the nylon‐6 film. As an immunoassay, the biochemical reaction between PANI‐Fe 2 O 3 ‐GA‐b‐NHS and BSA takes place on a surface of the nylon‐6 support film, which then translated into measurable resistance signal through pulse‐mode measurement. The in‐house developed nylon‐6 film, N‐16B, with 16 wt % nylon polymer and methanol as nonsolvent had demonstrated the fastest lateral wicking speed (1.07 mm s −1 ) and excellent protein immobilization capacity (1650 ± 85.84 μg cm −3 ), leading to the higher electrical current signal with a minimal resistance of electrochemical responses (0.57 ± 0.04 MΩ). This work reflects the importance of the physical characteristics of the nylon‐6 film that determines the sensitivity and effectiveness of an immunosensor. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 46741.