Premium
Functionalized polymeric membrane with aquaporin using click chemistry for water purification application
Author(s) -
He Yuan,
Hoi Hiofan,
Montemagno Carlo D.,
Abraham Sinoj
Publication year - 2018
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.46678
Subject(s) - polysulfone , membrane , azide , substrate (aquarium) , chemical engineering , click chemistry , aqueous solution , polymer chemistry , chemistry , conjugate , materials science , combinatorial chemistry , covalent bond , ultrafiltration (renal) , peptide , solvent , chromatography , organic chemistry , biochemistry , mathematical analysis , oceanography , mathematics , engineering , geology
A new type of aquaporin‐based biomimetic water purification membrane is fabricated using a propargyl functionalized β‐sheet peptide (FBP1). FBP1 is demonstrated to bind to aquaporin and form a stable protein–peptide complex in aqueous solution, as well as covalently conjugate with azide group. Substrate starting material, polysulfone (PSU), is modified with ∼1.05 azide group per repeating unit and casted into a thin film substrate with appropriate pore size structure using non‐solvent induced phase separation technique. Protein–peptide complex then is clicked onto the substrate using an in‐house built circulating system. The successful immobilization is verified by surface chemical composition analysis and surface morphology analysis. The preliminary salt rejection results show salt rejection improvement from 5% of control membrane to 12.5%. We thus demonstrate a promising method to conjugate a thin layer of membrane protein onto polymeric substrate and well maintain the functionality of membrane proteins. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135 , 46678.