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Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration
Author(s) -
Borges Felipe Azevedo,
de Barros Natan Roberto,
Garms Bruna Cambraia,
Miranda Matheus Carlos Romeiro,
Gemeinder Jose Lucio Padua,
RibeiroPaes João Tadeu,
Silva Rodrigo Ferreira,
de Toledo Karina Alves,
Herculano Rondinelli Donizetti
Publication year - 2017
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.45321
Subject(s) - biocompatibility , natural rubber , materials science , osteoblast , fourier transform infrared spectroscopy , mineralization (soil science) , cytotoxicity , scanning electron microscope , chemistry , chemical engineering , nuclear chemistry , polymer chemistry , composite material , in vitro , organic chemistry , biochemistry , nitrogen , engineering
Natural rubber latex (NRL) from Hevea brasiliensis is a colloidal system composed of cis ‐1,4‐polyisoprene. Its applications have grown due its angiogenic and wound healing activity. NRL has been used in guided bone regeneration as barrier, enhancing bone formation. However, there has been no study reported so far which shows its in vitro biocompatibility with osteoblasts. Thus, the aim of this work was to apply thermally induced phase separation under several temperatures to induce porosity in NRL; and test its mechanical properties, cytotoxicity, cell adhesion, and mineralization with MC3T3‐E1. Only biomembranes submitted at −20 and −10 °C presented porosity. Fourier transform infrared spectroscopy showed no change in cis ‐1,4‐isoprene spectra. Biomembranes were elastic (Young's modulus < 1 MPa). According to ISO10993–5, NRL showed no cytotoxicity. Cells adhered on the NRL and produced mineral matrix as analyzed by scanning electron microscopy–energy‐dispersive spectrometry, von kossa, and Fourier transform infrared spectroscopy. Cells on NRL presented higher alkaline phosphatase activity, however, mineralization showed no difference by alizarin red S dye extraction. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45321.