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Investigation on the interaction of collagen molecules in solution with different acetic acid concentrations
Author(s) -
Yang Huan,
Deng Yi,
Xu Songcheng,
Liu Wentao,
Li Guoying
Publication year - 2017
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.45255
Subject(s) - molecule , acetic acid , pyrene , chemistry , dynamic light scattering , fluorescence , hydrodynamic radius , solvent , biophysics , polymer chemistry , aqueous solution , materials science , organic chemistry , micelle , nanotechnology , nanoparticle , physics , quantum mechanics , biology
Collagen solutions in the presence of 0.1–2.0 M acetic acid (AA) were investigated to understand the interaction between collagen molecules and an acidic solvent. Fluorescence measurements of pyrene showed that the critical aggregation concentration (CAC) of collagen increased from 0.518 to 1.581 mg/mL for AA concentrations ranging from 0.1 to 2.0 M , indicating that the aggregated state of collagen molecules was associated with AA concentration. The size of the collagen aggregates, determined by dynamic light scattering, demonstrated that their disaggregation was enhanced with increasing electrostatic repulsion between the collagen chains. The variations in the intrinsic viscosity and Huggins coefficient depended on the molecular interaction among the collagen molecules. Furthermore, the increased AA concentration has a different influence on the rigidity of collagen molecules above and below the CAC. This observation was manifested by the changes in the morphology of collagen molecules observed by atomic force microscopy. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45255.