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Comparative study of visible light polymerized gelatin hydrogels for 3D culture of hepatic progenitor cells
Author(s) -
Greene Tanja,
Lin TsaiYu,
Andrisani Ourania M.,
Lin ChienChi
Publication year - 2017
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.44585
Subject(s) - photoinitiator , photopolymer , self healing hydrogels , ethylene glycol , gelatin , visible spectrum , materials science , polymerization , polymer chemistry , chemical engineering , chemistry , polymer , composite material , monomer , organic chemistry , optoelectronics , engineering
Photopolymerization techniques have been widely used to create hydrogels for biomedical applications. Visible light‐based photopolymerizations are commonly initiated by type II (i.e., noncleavage‐type) photoinitiator in conjunction with a coinitiator. On the other hand, type I photoinitiators (i.e., cleavage type) are rarely compatible with visible light‐based initiation due to their limited molar absorbability in the visible light wavelengths. Here, we report visible light initiated orthogonal photoclick crosslinking to fabricate gelatin‐norbornene and poly(ethylene glycol)‐tetra‐thiol hydrogels using either cleavage‐type (i.e., lithium acylphosphinate, LAP) or noncleavage‐type photoinitiator (i.e., eosin‐Y, EY) without the use of a coinitiator. Regardless of the initiator type, the step‐growth gelatin‐PEG hybrid hydrogels crosslinked and degraded similarly. While both systems exhibited similar cytocompatibility for hepatic progenitor HepaRG cells, gelation initiated by noncleavage‐type initiator EY afforded slightly higher degree of hepatic gene expression. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 44585.