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Fabrication of mPEGylated graphene oxide/poly(2‐dimethyl aminoethyl methacrylate) nanohybrids and their primary application for small interfering RNA delivery
Author(s) -
Sun Yu,
Zhou Junhui,
Cheng Qiang,
Lin Daoshu,
Jiang Qian,
Dong Anjie,
Liang Zicai,
Deng Liandong
Publication year - 2016
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.43303
Subject(s) - ethylene glycol , materials science , raft , chain transfer , biocompatibility , surface modification , graphene , polymer chemistry , methacrylate , methyl methacrylate , polymerization , conjugated system , oxide , lipofectamine , covalent bond , chemical engineering , polymer , nanotechnology , chemistry , organic chemistry , radical polymerization , engineering , metallurgy , composite material , recombinant dna , biochemistry , vector (molecular biology) , gene
Graphene oxide (GO)‐based nanohybrids were designed for small interfering RNA (siRNA) delivery for their high water dispensability, good biocompatibility, easily tunable surface functionalization, and particular optical properties. In this study, novel nanohybrids based on GO were fabricated. Methoxypoly(ethylene glycol) (mPEG) was covalently conjugated to GO via amide bonds. Then, poly(2‐dimethyl aminoethyl methacrylate) (PDMAEMA), which was synthesized via reversible addition–fragmentation chain transfer polymerization (RAFT) with 2‐(dodecyl thiocarbonothioyl thio)‐2‐methyl propionic acid (DTM) as the RAFT agent, was attached onto GO via physical interaction between DTM and GO. Compared with Lipofectamine 2000, the novel mPEG–GO/PDMAEMA nanohybrids showed comparable gene transfection efficiency and a low cytotoxicity. Moreover, the mPEG–GO/PDMAEMA nanohybrids showed enhanced optical properties compared to the original GO because of the presence of mPEG and PDMAEMA. Our work encouraged further exploration of the novel nanovector for combined photothermal and siRNA delivery. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43303.