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Fabrication and optimization of EGCG ‐loaded nanoparticles by high pressure homogenization
Author(s) -
Park Sung Jin,
Garcia Coralia V.,
Shin Gye Hwa,
Kim Jun Tae
Publication year - 2016
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.43269
Subject(s) - dispersity , zeta potential , homogenization (climate) , nanoparticle , epigallocatechin gallate , chitosan , particle size , chemical engineering , dpph , chemistry , gallate , surface charge , materials science , nuclear chemistry , antioxidant , polyphenol , polymer chemistry , nanotechnology , organic chemistry , biodiversity , ecology , engineering , biology
In this study, we investigated the effects of the number of high pressure homogenization cycles and alginate (AG)‐to‐chitosan (CS) ratio on the physicochemical properties (mean size, polydispersity index, surface charge, encapsulation efficiency, and free radical scavenging) of (‐)‐epigallocatechin‐3‐gallate (EGCG)‐loaded nanoparticles. Nanoparticles prepared with alginate and chitosan concentrations of 0.01% and three cycles of high pressure homogenization exhibited a small size (293 nm) and a zeta potential of +37.49 mV, and were thus considered to be optimal for encapsulation. The highest encapsulation efficiency of 80.1% was achieved by using an EGCG concentration of 100 µg/g, which also resulted in the highest 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activities of 81.8% and 69.3% for pH 2.6 and pH 6.9, respectively. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43269.