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Preparation and characterization of magnetic poly(styrene–glycidyl methacrylate) microspheres for highly efficient protein adsorption by two‐stage dispersion polymerization
Author(s) -
Zhao Xinwei,
Guan Yueping,
Xia Changfu,
Xia Tingting,
Qiu Xiaolin,
Wang Chuhang,
Guo Chen
Publication year - 2016
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.43005
Subject(s) - glycidyl methacrylate , dispersion polymerization , superparamagnetism , materials science , adsorption , polymerization , styrene , thermogravimetric analysis , scanning electron microscope , suspension polymerization , polymer chemistry , protein adsorption , magnetic nanoparticles , methacrylate , chemical engineering , copolymer , nuclear chemistry , nanoparticle , chemistry , polymer , nanotechnology , composite material , organic chemistry , magnetization , physics , quantum mechanics , magnetic field , engineering
Micrometer‐sized superparamagnetic poly(styrene–glycidyl methacrylate)/Fe 3 O 4 spheres were synthesized by two‐stage dispersion polymerization with modified hydrophobic Fe 3 O 4 nanoparticles, styrene (St), and glycidyl methacrylate (GMA). The morphology and properties of the magnetic Fe 3 O 4 –P (St‐GMA) microspheres were examined by scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, thermogravimetric analysis, and attenuated total reflectance. The average size of the obtained magnetic microspheres was 1.50 μm in diameter with a narrow size distribution, and the saturation magnetization of the magnetic microspheres was 8.23 emu/g. The magnetic Fe 3 O 4 –P (St‐GMA) microspheres with immobilized iminodiacetic acid–Cu 2+ groups were used to investigate the adsorption capacity and selectivity of the model proteins, bovine hemoglobin (BHb) and bovine serum albumin (BSA). We found that the adsorption capacity of BHb was as high as 190.66 mg/g of microspheres, which was 3.20 times greater than that of BSA, which was only 59.64 mg/g of microspheres as determined by high‐performance liquid chromatography. With a rather low nonspecific adsorption, these microspheres have great potential for protein separation and purification applications. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43005.