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Platelet lysate and adipose mesenchymal stromal cells on silk fibroin nonwoven mats for wound healing
Author(s) -
Theodora Chlapanidas,
Sara Perteghella,
Silvio Faragò,
Alessandra Boschi,
Giuseppe Tripodo,
Barbara Vigani,
Barbara Crivelli,
Sabrina Renzi,
Silvia Dotti,
Stefania Preda,
Mario Marazzi,
Maria Luisa Torre,
Maura Ferrari
Publication year - 2016
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.42942
Subject(s) - fibroin , mesenchymal stem cell , platelet lysate , wound healing , adipose tissue , stromal cell , tissue engineering , chemistry , microbiology and biotechnology , in vivo , materials science , biomedical engineering , silk , biology , pathology , immunology , medicine , composite material , biochemistry
In this work platelet lysate (PL) and adipose‐derived mesenchymal stromal cells (ASCs) seeded on nonwoven fibroin mats were in vitro and in vivo evaluated for tissue regenerative applications. Nonwoven mats obtained by a large scale water entanglement technique were characterized for their physico‐chemical properties. Results indicated a high purity of fibroin fibers, their stability after sterilization process and appropriate technological properties suitable for tissue engineering. Moreover, the scaffolds in vitro supported adhesion and migration of ASCs and the presence of PL improved the cell proliferation. The products were then applied on epithelial/dermal wounds carried out on the dorsal surface of rabbit: the skin reparative process was solved in 9 days, with a completely restitutio ad integrum of the epithelium in animals treated with PL alone; ASCs did not further improve the wound healing. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 42942.