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N ‐halamine‐modified polyglycolide (PGA) multifilament as a potential bactericidal surgical suture: In vitro study
Author(s) -
Umair Malik Muhammad,
Jiang Zhiming,
Safdar Waseem,
Xie Zhiwei,
Ren Xuehong
Publication year - 2015
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.42483
Subject(s) - chlorine , polyelectrolyte , copolymer , cationic polymerization , fourier transform infrared spectroscopy , titration , materials science , nuclear chemistry , chitosan , antibacterial activity , surface modification , chemistry , ultimate tensile strength , polymer chemistry , chemical engineering , composite material , bacteria , organic chemistry , biology , engineering , genetics , polymer
In this study, new cationic homopolymer and anionic copolymer were synthesized, and deposited onto polyglycolide sutures using a layer‐by‐layer assembly technique. The coated sutures were rendered antibacterial by chlorinating with dilute solution of household bleach solution at pH 7. The chlorination treatment transformed the NH groups of anionic copolymer into N ‐halamine structures. The N ‐halamine‐modified sutures were challenged with Staphylococcus aureus and Escherichia coli O157:H7 bacteria at different contact times. The suture with chlorine loading of 0.22% completely inactivated both bacterial strains in 30 min contact time. Fourier transform infrared spectroscopy, scanning electron microscopy, and analytical titration confirmed the successful deposition of the N ‐halamine multilayers. The effect of layer‐by‐layer coatings of polyelectrolytes on the chlorine loading and antibacterial efficacy of sutures was evaluated. The straight‐pull and knot‐pull strength tests performed on the sutures reported slight decline in tensile properties after chlorination treatment. The in vitro hemolysis and cytocompatibility tests revealed that the N ‐halamines‐based antibacterial sutures were biocompatible. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 42483.