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Random and aligned PLLA : PRGF electrospun scaffolds for regenerative medicine
Author(s) -
DíazGómez Luis,
Ballarin Florencia Montini,
Abraham Gustavo A.,
Concheiro Angel,
AlvarezLorenzo Carmen
Publication year - 2015
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.41372
Subject(s) - scaffold , nanofiber , materials science , mesenchymal stem cell , cell growth , regenerative medicine , tissue engineering , nanotechnology , electrospinning , platelet rich plasma , cell , biomedical engineering , biophysics , microbiology and biotechnology , chemistry , composite material , platelet , polymer , biochemistry , biology , immunology , engineering
ABSTRACT Random and aligned electrospun scaffolds were prepared combining poly( l ‐lactic acid) (PLLA) and activated platelet‐rich plasma (PRGF) at various proportions, with the aim of elucidating the role of nanofibers orientation and growth factors on cell attachment and proliferation. PRGF is released from scaffolds in a sustained way for at least 3 weeks, without an initial burst effect. Mesenchymal stem cells (MSCs) seeded on the random scaffolds present a polygonal and random orientation in any direction of the scaffold. On the other hand, aligned scaffolds are able to promote cell attachment and proliferation in the direction of the nanofibers. The incorporation of PRGF in the scaffolds enhances cell proliferation for at least 2 weeks. Overall, aligned electrospun PLLA : PRGF scaffolds can encapsulate growth factors at relatively large proportions and sustain their release to enhance cell attachment and proliferation as well as eliciting cell alignment. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41372.