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The effect of BMP‐2 and VEGF loading of gelatin‐pectin‐BCP scaffolds to enhance osteoblast proliferation
Author(s) -
Amirian Jhaleh,
Linh Nguyen Thuy Ba,
Min Young Ki,
Lee ByongTaek
Publication year - 2015
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.41241
Subject(s) - gelatin , bone morphogenetic protein 2 , materials science , scanning electron microscope , osteoblast , chemistry , chemical engineering , scaffold , vascular endothelial growth factor , fourier transform infrared spectroscopy , tissue engineering , angiogenesis , pectin , cell growth , vegf receptors , biophysics , biomedical engineering , composite material , biochemistry , medicine , cancer research , engineering , in vitro , biology
A composite scaffold of gelatine (Gel)‐pectin (Pec)‐biphasic calcium phosphate (BCP) was successfully fabricated. Growth factors such as bone morphogenetic protein‐2 (BMP‐2) and vascular endothelial growth factor (VEGF) were loaded into the Gel‐Pec‐BCP hydrogel scaffolds by freeze‐drying. The surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive and X‐ray diffraction spectroscopy. The results obtained from Fourier transform infrared spectroscopy and quantitative measurements showed successfully loading of BMP‐2 and VEGF into the Gel‐Pec‐BCP hydrogel scaffolds. In addition MC3T3‐E1 preosteoblasts were cultivated on the three types of scaffolds to investigate the effects of BMP‐2 and VEGF on cell viability and proliferation. The Gel‐Pec‐BCP scaffolds loaded with VEGF and BMP‐2 demonstrated more cell spreading and proliferation compared to those of the Gel‐Pec‐BCP scaffolds. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41241.