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A molecularly imprinted polymer as artificial receptor for the detection of indole‐3‐carbinol
Author(s) -
Scorrano Sonia,
Mergola Lucia,
Del Sole Roberta,
Lazzoi Maria Rosaria,
Vasapollo Giuseppe
Publication year - 2014
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.40819
Subject(s) - molecularly imprinted polymer , solid phase extraction , elution , sorbent , molecular imprinting , chemistry , acetonitrile , polymer , combinatorial chemistry , monomer , indole test , chromatography , selectivity , extraction (chemistry) , adsorption , organic chemistry , catalysis
ABSTRACT Molecularly imprinting polymer technology is used to prepare a molecularly imprinted polymer (MIP) for the selective recognition of indole‐3‐carbinol (I3C), a chemopreventive and chemotherapeutic phytochemical associated with the anticancer activities of cruciferous vegetables. Prepolymerization study via nuclear magnetic resonance technique is done to choose the best functional monomer that establishes more interaction with the template. The prepared MIP is tested before in batch experiments and subsequently used as solid‐phase extraction sorbent for the selective detection of I3C from standard solutions. In order to verify the selectivity of the MIP, the binding of structurally related compounds, such as indole‐3‐acetonitrile, teophylline, and tryptophan, on the polymer is investigated. The experiments indicate that the MIP is highly selective for I3C with an association constant of K a = (1.37 ± 0.07) × 10 3 M −1 . Standard mixture solution loaded on MIP‐SPE cartridge give a recovery of 95% for I3C, while the other compounds are totally eluted during washing step. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40819.