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“One‐pot” synthesis of well‐defined functional copolymer and its application as tumor‐targeting nanocarrier in drug delivery
Author(s) -
Wang Chenhong,
Qiao Lei,
Yan Husheng,
Liu Keliang
Publication year - 2014
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.40405
Subject(s) - nanocarriers , peg ratio , conjugated system , copolymer , hela , chemistry , polymer chemistry , nanoparticle , polyethylene glycol , drug delivery , click chemistry , atom transfer radical polymerization , combinatorial chemistry , superparamagnetism , polymer , materials science , organic chemistry , nanotechnology , biochemistry , cell , finance , economics , magnetization , physics , quantum mechanics , magnetic field
ABSTRACT A one‐pot synthesis is developed for PEG 600 ‐ b ‐poly(glycerol monoacrylate) (PEG 600 ‐ b ‐PGA), by which folate and superparamagnetic iron oxide nanoparticles (SPIONs) are assembled to form folic acid‐conjugated magnetic nanoparticles (FA‐MNPs) as a tumor targeting system. The synthesis consists of a “click” reaction and atom transfer radical polymerization (ATRP) to obtain the well‐defined furan‐protected maleimido‐terminated PEG 600 ‐ b ‐poly(solketal acrylate) (PEG 600 ‐ b ‐PSA) copolymer. After deprotection, the key copolymer N ‐maleimido‐terminated PEG 600 ‐ b ‐PGA is successfully conjugated with thiol derivatives of folate and FITC, respectively. FA‐MNPs are developed by assembling of the resulting polymer FA‐PEG 600 ‐ b ‐PGA with SPIONs, and characterized for their size, surface charge, and superparamagnetic properties. To investigate the cellular uptake of the nanoparticles by Hela cells and φ2 cells using fluoresce technique, FA‐FITC‐MNPs are also obtained by assembling of FA‐PEG 600 ‐ b ‐PGA, FITC‐PEG 600 ‐ b ‐PGA with SPIONs. Qualitative and quantitative determinations of FA‐FITC‐MNPs show that the particles specifically internalized to Hela cells. No significant cytotoxicity is observed for these two kinds of cell lines. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40405.