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Assessment of cell proliferation in salt‐leaching using powder (SLUP) scaffolds with penetrated macro‐pores
Author(s) -
Cho Yong Sang,
Hong Myoung Wha,
Kim Young Yul,
Cho YoungSam
Publication year - 2014
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.40240
Subject(s) - mold , leaching (pedology) , materials science , scaffold , porosity , macropore , fabrication , polycaprolactone , compressive strength , composite material , chemical engineering , chemistry , biomedical engineering , mesoporous material , medicine , biochemistry , environmental science , alternative medicine , polymer , pathology , soil science , soil water , engineering , catalysis
In this study, a salt‐leaching using powder (SLUP) scaffold with penetrated macropores was proposed to enhance cell proliferation. A SLUP scaffold is a salt‐leaching scaffold with an arbitrary pore configuration. Although SLUP scaffolds have several advantage over traditional salt‐leaching scaffolds, the cell ingrowth might be poor compared with solid freeform fabrication scaffolds, which have well‐interconnected pores. We therefore proposed SLUP scaffolds with penetrated macropores to assist the cell ingrowth. First, polycaprolactone (PCL) powders with a grain size of 63–100 μm and NaCl powders with a grain size of 100–180 μm were prepared. Next, a uniformly perforated mold was fabricated using an rapid prototyping (RP) system. Then, 500‐, 820‐, or 1200‐μm‐diameter needles were inserted into the holes of the RP mold. Subsequently, the mold was filled with a mixed powder of PCL/NaCl (30 : 70 vol %). The mold was then heated in the oven at 100°C for 30 min, and both the needles and the mold were removed from the PCL/NaCl mixture. Then, the PCL/NaCl mixture was soaked in DI water for 24 h to leach out NaCl particles and dried in a vacuum desiccator for 24 h. The porosity of fabricated scaffolds was calculated using a simple equation, and the compressive stiffness was measured using a universal testing machine. Moreover, each scaffold (10 × 10 × 10 mm 3 ) was seeded with 100,000 Saos‐2 cells and cultured for 14 days. The cell proliferation characteristics were assessed using a CCK‐8 assay at 1, 7, and 14 days for comparison with the control scaffolds, that is, the SLUP scaffolds with no penetrated macropores. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40240.