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Influence of chitosan derivatization on its physicochemical characteristics and its use as enzyme support
Author(s) -
Urrutia Paulina,
Bernal Claudia,
Escobar Sindy,
Santa Cristiam,
Mesa Monica,
Wilson Lorena,
Illanes Andres
Publication year - 2014
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.40171
Subject(s) - chitosan , epichlorohydrin , glutaraldehyde , derivatization , thermal stability , bacillus circulans , immobilized enzyme , chemistry , biocatalysis , polymer , nuclear chemistry , polymer chemistry , chromatography , organic chemistry , enzyme , mass spectrometry , catalysis , reaction mechanism
Chitosan was derivatized by two methodologies for analyzing their effect on chitosan physicochemical characteristics and its applicability as carrier for Bacillus circulans β‐galactosidase immobilization. Glutaraldehyde (GA) and epichlorohydrin (EPI) were used for crosslinking and activation of chitosan, producing the corresponding supports (C‐GA and C‐EPI‐EPI) after a one‐step and a two‐step process, respectively. The spherical shape and mean diameter of chitosan particles was not significantly affected by polymer derivatization, while Fourier transform infrared analysis showed that in both cases, chitosan polymer was chemically modified. TGA analysis indicated that C‐EPI‐EPI was the most thermally stable. The high degree of activation of C‐EPI‐EPI (586 μmol of aldehydes/g) resulted in the highest loss of activity during immobilization; hence a support with 100 μmol of aldehydes/g was produced (C‐EPI‐EPI 100 ). The highest expressed activity (89.3 IU/g) was obtained with the enzyme immobilized in C‐GA, while the biocatalyst with highest thermal stability at 60°C was obtained with C‐EPI‐EPI 100 (half‐life was 84‐fold higher than the one of the soluble enzyme). The best compromise between biocatalyst expressed activity and thermal stability corresponded to β‐galactosidase immobilized in C‐EPI‐EPI 100 . According to this study, chitosan derivatized with EPI is a thermally stable carrier appropriate for producing highly stable immobilized B. circulans β‐galactosidase. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40171.