Premium
Magnetic polymeric nanoparticles functionalized by mannose‐rhodamine conjugate for detection of E. coli
Author(s) -
Trungkathan Samon,
Polpanich Duangporn,
Smanmoo Srung,
Tangboriboonrat Pramuan
Publication year - 2014
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.40012
Subject(s) - mannose , conjugate , rhodamine b , magnetic nanoparticles , nanoparticle , fluorescence , rhodamine , mannose receptor , materials science , styrene , substrate (aquarium) , benzene , chemistry , nuclear chemistry , acrylic acid , polymer chemistry , copolymer , nanotechnology , polymer , organic chemistry , biochemistry , catalysis , in vitro , mathematical analysis , mathematics , macrophage , oceanography , photocatalysis , quantum mechanics , physics , geology
Mannose‐rhodamine (Rh) conjugate (80% yield) was synthesized in a one‐pot reaction and immobilized onto magnetic polymeric nanoparticles (MPNP; 43% magnetic content) of poly(styrene/divinyl benzene/acrylic acid). The resulting nanoparticles contained MPNP as a substrate, mannose as an E. coli receptor and Rh as a fluorescent signaling unit. TEM imaging clearly demonstrated that multiple mannose‐Rh MPNPs could be captured by E. coli strain ORN178. The fluorescent signal from captured nanoparticles was emitted at 580 nm. These results indicate that mannose‐Rh MPNP offers a simple and rapid strategy for bacterial detection. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2014 , 131 , 40012.