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Structural and functional changes of silk fibroin scaffold due to hydrolytic degradation
Author(s) -
Farokhi Mehdi,
Mottaghitalab Fatemeh,
Hadjati Jamshid,
Omidvar Ramin,
Majidi Mohammad,
Amanzadeh Amir,
Azami Mahmoud,
Tavangar Seyed Mohammad,
Shokrgozar Mohammad Ali,
Ai Jafar
Publication year - 2014
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.39980
Subject(s) - fibroin , fourier transform infrared spectroscopy , biocompatibility , scanning electron microscope , raman spectroscopy , alkaline phosphatase , silk , materials science , glutaraldehyde , infrared spectroscopy , biomaterial , nuclear chemistry , chemistry , chemical engineering , composite material , nanotechnology , chromatography , organic chemistry , physics , optics , metallurgy , enzyme , engineering
In this study, hydrolytic degradation of silk fibroin (SF) in Phosphate buffer saline (PBS) after 12 weeks incubation was investigated. Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy and X‐ray diffraction (XRD) patterns have confirmed the transition from crystalline β‐sheet to random coil in treated SF. A decrease in adhesion force and surface Young's modulus were observed using atomic force microscopy (AFM). Structural changes were further confirmed using scanning electron microscopy (SEM). Biocompatibility and alkaline phosphatase production of osteoblast cells were decreased significantly in treated SF scaffold. Moreover, a significant decrease in mRNA level of collagen type I and osteopontin compared with fresh SF scaffold was observed. Finally, structural and biological characteristics of SF scaffold could alter in PBS. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2014 , 131 , 39980.