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Extraction of protein from skim natural rubber latex using PEG as a surfactant via low speed centrifugation and continuous flow
Author(s) -
Danwanichakul Duangkamol,
Rattanaphan Ornthana,
Srisatjang Jirarat,
Danwanichakul Panu
Publication year - 2014
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.39900
Subject(s) - extraction (chemistry) , chromatography , natural rubber , polyethylene glycol , centrifugation , pulmonary surfactant , peg ratio , materials science , chemistry , composite material , biochemistry , finance , economics , organic chemistry
Protein extractions from skim natural rubber latex using 3 %w/v polyethylene glycol (PEG6000) via both low speed centrifugation and continuous flow were investigated. In centrifugal extraction, when the speed was 1000 rpm, the extractable protein (EP) content in serum increased with processing time from 5 to 30 min and when the time was fixed at 5 min, EP content increased with centrifugal speed. In addition, further washing deproteinized chips with 2 %w/v SDS solution could remove proteins with efficiencies corresponding to the efficiencies of protein removals in latex phase, implying the role of PEG in protein reduction in both steps. In continuous flow extraction, EP content increased with increasing Reynolds number or increasing mean residence time of the flow to a maximum and then dropped. The efficiencies of the centrifugal extraction and continuous flow extraction were 55.2 and 33.7%, respectively. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 39900.