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Production of 3D scaffolds applied to tissue engineering using chitosan swelling as a porogenic agent
Author(s) -
Mendonça Roberta Helena,
de Oliveira Meiga Taíla,
da Costa Marysilvia Ferreira,
da Silva Moreira Thiré Rossana Mara
Publication year - 2012
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.38735
Subject(s) - swelling , chitosan , scaffold , materials science , cancellous bone , porosity , tissue engineering , matrix (chemical analysis) , scanning electron microscope , biomedical engineering , drug delivery , chemical engineering , compressive strength , morphology (biology) , composite material , nanotechnology , anatomy , medicine , genetics , biology , engineering
The preparation of 3D scaffolds using poly(3‐hydroxybutyrate) (PHB) and chitosan (CTS) has been proposed because of the favorable interactions of these materials with mammalian cells in particular osteoblasts (HOB). In this work, a new method of producing porous 3D scaffolds based on the swelling of a dense matrix of PHB and CTS was developed. Scanning electron microscopy images showed the formation of an interconnected porous CTS structure inside a PHB structure. The pore formation was attributed to the swelling of CTS. The scaffolds presented 70% porosity, heterogeneous pore‐size distribution, and a compressive modulus equal to (13.07 ± 0.014) MPa, which is close to that of cancellous bone. In vitro cytotoxicity assays indicate that the scaffold was not cytotoxic. Its morphology, chemical, and physical properties indicate that the scaffold produced by this new methodology has potential applications in bone‐tissue engineering, with drug‐delivery capabilities. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013