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Synthesis of star polymer poly(ethylene glycol) 3 –poly( N,N ‐dimethyl acrylamide) and its application in protein resistance and separation
Author(s) -
Xing Jinxing,
Tan Lin,
Cao Fuhu,
Wang Yanmei
Publication year - 2012
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.38559
Subject(s) - ethylene glycol , peg ratio , atom transfer radical polymerization , polymer , copolymer , protein adsorption , materials science , polymer chemistry , gel permeation chromatography , chemical engineering , adsorption , quartz crystal microbalance , acrylamide , polymerization , radical polymerization , chemistry , organic chemistry , composite material , finance , engineering , economics
A star polymer composed of three poly(ethylene glycol) (PEG) arms and one poly( N,N ‐dimethyl acrylamide) (PDMA) arm (PEG 3 –PDMA) was synthesized by amidation and atom‐transfer radical polymerization. The structure of PEG 3 –PDMA was confirmed by 1 H‐NMR and gel permeation chromatography results. The surface adsorption and protein‐resistance behaviors of the star polymer PEG 3 –PDMA, diblock copolymer PEG–PDMA, and homopolymer PEG were investigated by a quartz crystal microbalance with dissipation. The results indicate that the PEG 3 –PDMA coating could reduce protein adsorption to 13% at least, more effectively than the PEG–PDMA coating; this indicated that the protein‐resistance properties depended on the PEG chain density and surface coverage. If PEG 3 –PDMA were to be used as the physical coating in capillary zone electrophoresis, it could yield a well‐suppressed eletroosmotic flow with greater stability and separate proteins with a lower relative standard deviration (RSD) of protein migration time and a higher separation efficiency than a bare fused‐silica capillary in a broad pH range. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013