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Extrusion‐based differences in two types of nylon 6 capillary‐channeled polymer (C‐CP) fiber stationary phases as applied to the separation of proteins via ion exchange chromatography
Author(s) -
SchadockHewitt Abby J.,
Pittman Jennifer J.,
Stevens Kathryn A.,
Marcus R. Kenneth
Publication year - 2012
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.38509
Subject(s) - nylon 6 , crystallinity , fiber , extrusion , differential scanning calorimetry , polymer , fourier transform infrared spectroscopy , nylon 66 , materials science , capillary action , analytical chemistry (journal) , polymer chemistry , chemistry , chromatography , chemical engineering , polyamide , composite material , physics , engineering , thermodynamics
Nylon 6 capillary‐channeled polymer (C‐CP) fibers used as the stationary phase in high‐performance liquid chromatography protein separations have been investigated. A difference in chromatographic behavior between two (presumably identical) nylon 6 C‐CP fibers, designated simply as nylon 6A and nylon 6B, reveals unknown differences in their chemical and physical nature as proteins are less retained on the latter. The possible differences are evaluated through several test methods, including Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), mechanical testing, MALDI‐TOF mass spectrometry, an organic reaction with ninhydrin, and dilute solution viscometry. Nylon 6B is shown to be a larger perimeter, stronger fiber, yet in a 200 mm, 0.8 mm i.d. column having an interstitial fraction of ∼0.6, it has an available surface area of ∼189 cm 2 , while nylon 6A is almost double that at ∼374 cm 2 . It is revealed that when in amorphous form, the base polymers have very comparable FTIR and DSC characteristics. When in their solid, extruded fiber form there are significant differences found in crystallinity, tensile strength, molecular weight distribution, and end group density. Nylon 6A is a lower molecular weight fiber, which results in ∼1.3× more amine end groups than nylon 6B. It is the end group density that ultimately determines the separation qualities. These results imply that both a difference in base nylon 6 molecular weight and also the fiber extrusion process have a significant influence on the performance of nylon 6 C‐CP fiber separation of proteins via ion exchange chromatography. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013