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Electrochemical polymerization of 2‐thiophen‐3‐yl‐malonic acid for biosensor application
Author(s) -
Celik Gamze,
Eren Esin,
Uygun Aysegul
Publication year - 2012
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.38205
Subject(s) - glucose oxidase , glutaraldehyde , supporting electrolyte , biosensor , immobilized enzyme , electrochemistry , fourier transform infrared spectroscopy , perchlorate , electrode , malonic acid , nuclear chemistry , polymer chemistry , polymerization , electrolyte , acetonitrile , chemistry , materials science , organic chemistry , chemical engineering , polymer , enzyme , ion , biochemistry , engineering
Poly(2‐thiophene‐3‐yl‐malonic acid) (P3TMA) was synthesized electrochemically using tetrabutylammonium perchlorate (TBAClO 4 ) as electrolyte into acetonitrile (ACN) for glucose biosensor application. The properties of P3TMA were investigated using FTIR, SEM, and electrochemical measurements. The glucose oxidase enzyme (GOD) was chemically immobilized onto P3TMA modified electrode using glutaraldehyde as crosslinking. It was observed that response current of P3TMA/GOD enzyme electrode increased linearly with loading glucose concentration. Michaelis Menten constant ( K m ) of P3TMA/GOD enzyme electrode was calculated as 0.03517 m M . FTIR and SEM analyses were used to confirm immobilization of GOD onto surface of P3TMA‐modified electrode. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013