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Extraction and in vitro antioxidant activity of mopan persimmon polysaccharide
Author(s) -
Chen Yilun,
Zhang Jianjun,
Li Chunmei,
Chen Zhiqiang,
Jia Le
Publication year - 2011
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.35200
Subject(s) - sephadex , chemistry , polysaccharide , chromatography , extraction (chemistry) , column chromatography , antioxidant , absorbance , cellulose , dpph , hydroxyl radical , nuclear chemistry , organic chemistry , enzyme
The optimal extraction parameters of Mopan persimmon polysaccharide (MPP) were ultrasonic power 900 W, ultrasonic treatment time 3 min, and water addition 2000%. Four subfractions (MPP‐1, MPP‐2b, MPP‐3, and MPP‐4b) were separated by DEAE–52 cellulose anion‐exchange column chromatography and then Sephadex G‐200 gel column chromatography. The in vitro scavenging effects of MPP and MPP‐4b at a dosage of 10 g/L on hydroxyl radical were 94.15 ± 8.27% and 96.61 ± 8.06%, respectively. The 1,1‐diphenyl‐2‐picrylhydrazyl inhibition percentages of two polysaccharides fractions were 93.37 ± 7.66% and 70.79 ± 6.15%, respectively. The reducing power (absorbance at 700 nm) of MPP and MPP‐4b were 4.31 ± 0.26 and 5.09 ± 0.33, respectively. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012