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Antibody purification from human plasma by metal‐chelated affinity membranes
Author(s) -
Yavuz Handan,
Bereli Nilay,
Armutçu Canan,
Yılmaz Fatma,
Denizli Adil
Publication year - 2011
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.34672
Subject(s) - membrane , adsorption , chelation , metal ions in aqueous solution , chemistry , metal , polymerization , nuclear chemistry , polymer chemistry , methacrylate , chromatography , elution , inorganic chemistry , organic chemistry , biochemistry , polymer
The aim of this study is to investigate in detail the feasibility of poly(2‐hydroxyethyl methacrylate‐ N ‐methacryloyl‐( L )‐histidine methyl ester), PHEMAH membranes for purification of immunoglobulin G (IgG) from human plasma. PHEMAH membranes were prepared by photo‐polymerization technique. Then, Zn 2+ , Ni 2+ , Co 2+ , and Cu 2+ ions were chelated directly on the PHEMAH membranes. Elemental analysis assay was performed to determine the nitrogen content and polymerized MAH was calculated as 168.5 μmol/g. The nonspecific IgG adsorption onto the plain PHEMA membranes was negligible (about 0.25 mg/mL). A remarkable increase in the IgG adsorption capacities were achieved from human plasma with PHEMAH membranes (up to 68.4 mg/mL). Further increase was observed with the metal‐chelated PHEMAH membranes (up to 118 mg/mL). The metal‐chelate affinity membranes allowed the one‐step separation of IgG from human plasma. The binding range of metal ions for surface histidines from human plasma followed the order: Cu 2+ > Ni 2+ > Zn 2+ > Co 2+ . Adsorbed IgG was eluted using 250 m M EDTA with a purity of 94.1%. IgG molecules could be repeatedly adsorbed and eluted with the metal‐chelated PHEMAH membranes without noticeable loss in their IgG adsorption capacity. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012