Premium
Hyperbranched PEI grafted by hydrophilic amino acid segment poly[ N ‐(2‐hydroxyethyl)‐ L ‐glutamine] as an efficient nonviral gene carrier
Author(s) -
Chen Jie,
Tian Huayu,
Guo Zhaopei,
Lin Lin,
Dong Xuan,
Zhu Xiaojuan,
Chen Xuesi
Publication year - 2011
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.34562
Subject(s) - polyethylenimine , gene delivery , transfection , zeta potential , hela , cytotoxicity , in vivo , in vitro , microbiology and biotechnology , materials science , glutamine , biophysics , chemistry , nuclear chemistry , biochemistry , biology , nanotechnology , amino acid , nanoparticle , gene
A polyethylenimine‐poly(hydroxyethyl glutamine) copolymer (PEI‐PHEG) was designed and synthesized as a gene delivery system. The molecular structure of PEI‐PHEG was characterized using nuclear magnetic resonance. Moreover, PEI‐PHEG/ p DNA complexes were fabricated and characterized by gel retardation assay, particle size analysis, and zeta potential analysis. The transfection efficiency and cytotoxicity of PEI‐PHEG were evaluated using human cervical carcinoma (HeLa), human embryonic kidney (HEK293), and murine colorectal adenocarcinoma (CT26) cells in vitro . The results show that PEI‐PHEG could effectively form positively charged nano‐sized particles with p DNA; the particle size was in a range of 130.2 to 173.0 nm and the zeta potential was in a range of 27.6 to 41.0 mV. PEI‐PHEG exhibited much lower cytotoxicity and higher gene transfection efficiency than PEI‐25K with different cell lines in vitro . An animal test was also conducted on a Lewis Lung Carcinoma tumor model in C57/BL6 mice by using subcutaneous intratumoral administration. The results show that in vivo transfection efficiency of PEI‐PHEG was improved greatly compared with that of commercial PEI‐25K. These results demonstrate that PEI‐PHEG can be a potential nonviral vector for gene delivery systems both in vitro and in vivo . © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012