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Antifungal activity of chitosan against Fusarium oxysporum f. sp. cubense
Author(s) -
AlHetar M. Y.,
Zainal Abidin M. A.,
Sariah M.,
Wong M. Y.
Publication year - 2010
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.33455
Subject(s) - chitosan , fusarium oxysporum f.sp. cubense , mycelium , spore germination , fusarium oxysporum , spore , food science , hypha , potato dextrose agar , chemistry , growth inhibition , nuclear chemistry , agar , horticulture , botany , in vitro , biology , fusarium wilt , biochemistry , bacteria , genetics
The in vitro antifungal activity of chitosan against Fusarium oxysporum f. sp. cubense Race 4 (FocR4) the causal agent of banana wilt was investigated. Chitosan at all concentrations tested reduced the hyphal growth of FocR4 on potato dextrose agar media and recording maximum inhibition of 76.36% at 8 mg/mL. The inhibitory effect was found to increase as chitosan concentration increases. The 50% effective concentration value was estimated by probit analysis, and it was 1.4 mg/mL. Chitosan was more effective in potato dextrose broth where it completely inhibited the mycelial growth of FocR4 at all concentrations tested. Chitosan inhibited the sporulation of FocR4 by a maximum of 96.53% at 8 mg/mL chitosan, and 100% inhibition for spore germination was recorded at all concentrations tested. Chitosan at concentrations of more than 1.6 mg/mL was also found to induce morphological changes in FocR4 characterized by agglomeration of hyphae, abnormal shapes, vesicles, or empty cells devoid of cytoplasm in the mycelia. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011