Premium
Electrospun poly( L ‐lactide)/poly(ε‐caprolactone) blend fibers and their cellular response to adipose‐derived stem cells
Author(s) -
Liao GuiYing,
Chen Liang,
Zeng XiaoYong,
Zhou XingPing,
Xie XiaoLin,
Peng EJun,
Ye ZhangQun,
Mai YiuWing
Publication year - 2010
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.33398
Subject(s) - materials science , caprolactone , electrospinning , biocompatibility , tissue engineering , crystallinity , polyester , scaffold , polymer blend , membrane , polymer , polycaprolactone , composite material , chemical engineering , polymer chemistry , biomedical engineering , copolymer , chemistry , medicine , biochemistry , metallurgy , engineering
Polymer blending is one of the most effective methods for providing new, desirable biocomposites for tissue‐engineering applications. In this study, electrospun poly( L ‐lactide)/poly(ε‐caprolactone) (PLLA/PCL) blend fibrous membranes with defect‐free morphology and uniform diameter were optimally prepared by a 1 : 1 ratio of PLLA/PCL blend under a solution concentration of 10 wt %, an applied voltage of 20 kV, and a tip‐to‐collector distance of 15 cm. The fibrous membranes also showed a porous structure and high ductility. Because of the rapid solidification of polymer solution during electrospinning, the crystallinity of electrospun PLLA/PCL blend fibers was much lower than that of the PLLA/PCL blend cast film. To obtain an initial understanding of biocompatibility, adipose‐derived stem cells (ADSCs) were used as seed cells to assess the cellular response, including morphology, proliferation, viability, attachment, and multilineage differentiation on the PLLA/PCL blend fibrous scaffold. Because of the good biocompatibility and nontoxic effect on ADSCs, the PLLA/PCL blend electrospun fibrous membrane provided a high‐performance scaffold for feasible application in tissue engineering using ADSCs. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011