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DNA adsorption on poly( N , N ‐dimethylacrylamide)‐grafted chitosan hydrogels
Author(s) -
Başer Burcu,
Demirel Gökçen Birlik,
Caykara Tuncer
Publication year - 2010
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.33029
Subject(s) - self healing hydrogels , chitosan , adsorption , polymer chemistry , grafting , dispersity , gel permeation chromatography , fourier transform infrared spectroscopy , nuclear chemistry , polymerization , chemistry , monomer , radical polymerization , chemical engineering , organic chemistry , polymer , engineering
Abstract In this study, poly( N,N ‐dimethylacrylamide) grafted chitosan (PDMAAm‐ g ‐CT) hydrogels were prepared for deoxyribonucleic acid (DNA) adsorption. Instead of directly grafting the N,N ‐dimethylacrylamide (DMAAm) monomer onto the chitosan (CT) chains, poly( N,N ‐dimethylacrylamide) with carboxylic acid end group (PDMAAm‐COOH) was firstly synthesized by free‐radical polymerization using mercaptoacetic acid (MAAc) as the chain‐transfer agent and then grafted onto the CT having amino groups. The synthesis of PDMAAm‐COOH and its grafting onto the CT chains were confirmed by attenuated total reflectance Fourier transform infrared (ATR‐FTIR) spectroscopy. From gel permeation chromatography measurements, the number‐average molecular weight ( M n ) and polydispersity index of PDMAAm‐COOH were found as 2400 g/mol and 2.3, respectively. The PDMAAm‐ g ‐CT hydrogels were utilized as the adsorbents in DNA adsorption experiments conducted at +4°C in a trisEDTA solution of pH 7.4. The hydrogels produced with higher PDMAAm‐COOH content exhibited higher DNA adsorption capacity. The DNA adsorption capacity up to 4620 μg DNA/g dry gel could be achieved with the PDMAAm‐ g ‐CT hydrogels prepared in 80.0 wt % PDMAAm‐COOH feed concentration. This value is approximately seven times higher than that of CT alone. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011