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Synthesis of a novel histidine‐targeting poly(ethylene glycol) and modification of lysozyme
Author(s) -
Sun Jingjing,
Song Xiaoda,
Tian Hong,
Jin Yuhao,
Gao Xiangdong,
Yao Wenbing
Publication year - 2010
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.32908
Subject(s) - lysozyme , ethylene glycol , diazo , histidine , derivative (finance) , peg ratio , chemistry , sodium nitrite , polymer chemistry , combinatorial chemistry , organic chemistry , enzyme , biochemistry , finance , financial economics , economics
A simple and highly efficient method was developed to prepare a synthetic poly(ethylene glycol) (PEG) derivative, which can be used for conjugation to histidine residue. This derivative reacted with sodium nitrite and the intermediate 4‐diazo‐N‐mPEG‐benzamide proved to have a good performance for histidine modification of lysozyme. Then the pegylated lysozyme was separated and its bacteriolytic activities were determined. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011