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Entrapment of urease in poly(1‐vinyl imidazole)/poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) network
Author(s) -
Şenel Mehmet,
Çevik Emre,
Abasıyanık M. Fatih,
Bozkurt Ayhan
Publication year - 2010
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.32896
Subject(s) - imidazole , urease , fourier transform infrared spectroscopy , dielectric spectroscopy , interpenetrating polymer network , polymer , polymer chemistry , immobilized enzyme , scanning electron microscope , materials science , nuclear chemistry , thermal stability , chemical engineering , chemistry , organic chemistry , enzyme , composite material , electrochemistry , electrode , engineering
In this article, urease was immobilized in a conducting network via complexation of poly(1‐vinyl imidazole) (PVI) with poly(2‐acrylamido‐2‐methyl‐1‐propanesulfonic acid) (PAMPS). The preparation method for the polymer network was adjusted by using Fourier transform infrared (FTIR) spectroscopy. A scanning electron microscope (SEM) study revealed that enzyme immobilization had a strong effect on film morphology. The proton conductivity of the PVI/PAMPS network was measured via impedance spectroscopy, under humidified conditions. The basic characteristics (Michealis‐Menten constants, pH opt , pH stability , T opt , T stability , reusability, and storage stability) of the immobilized urease were determined. The obtained results showed that the PAA/PVI polymer network was suitable for enzyme immobilization. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
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