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Interaction between the fluorinated amphiphilic copolymer poly(2,2,3,4,4,4‐hexafluorobutyl methacrylate)‐ graft ‐poly(SPEG) and DNA
Author(s) -
Li Ling,
Xiong Shengdong,
Wang Yingxi,
Song Gongwu,
Wu Shuilin,
Chu Paul K.,
Xu Zushun
Publication year - 2010
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.32339
Subject(s) - copolymer , methacrylate , materials science , amphiphile , polymer chemistry , dynamic light scattering , spectroscopy , chemical engineering , nanotechnology , polymer , composite material , physics , quantum mechanics , nanoparticle , engineering
A synthesized copolymer, synthesized from HFMA (hexaflurobutyl methacrylate) and SPEG (PHFMA‐ g ‐PSPEG), was synthesized. PHFMA‐ g ‐PSPEG intercalated to the DNA base pair via a strong hydrophobic force, and this was conformed by ultraviolet spectroscopy, transmittance measurements, micropolarity measurements, resonance light scattering (RLS) spectroscopy, and particle size measurements. The copolymer was used as a new probe to detect DNA according to the RLS technique. The hydrophobic interaction between PHFMA‐ g ‐PSPEG and DNA significantly enhanced the RLS signal, and the enhanced RLS intensity at 422 nm was proportional to the nucleic acid concentration within the range of 0.09–0.90 mg/L with a detection limit (3σ) of 4.0 μg/L. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010