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Coimmobilization of malic enzyme and alanine dehydrogenase on organic–inorganic hybrid gel fibers and the production of L ‐alanine from malic acid using the fibers with coenzyme regeneration
Author(s) -
Nakane Koji,
Suye ShinIchiro,
Ueno Tomoya,
Ohno Yoko,
Ishikawa Tomoko,
Ogihara Takashi,
Ogata Nobuo
Publication year - 2010
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.31783
Subject(s) - malic acid , malic enzyme , biochemistry , alanine , enzyme , chemistry , immobilized enzyme , nicotinamide adenine dinucleotide , dehydrogenase , nad+ kinase , citric acid , amino acid
Malic enzyme (EC 1.1.1.39) and alanine dehydrogenase (EC 1.4.1.1) were entrap‐immobilized on hybrid gel fibers of cellulose acetate (CA) and zirconium (Zr) alkoxide by air‐gap wet spinning. The production of L ‐alanine from malic acid with coenzyme regeneration was examined with the enzymes immobilized on the fibers. The productivity of L ‐alanine of the immobilized enzymes decreased to approximately one‐fifth of that of free enzymes, but the CA–Zr‐fiber‐immobilized enzymes retained a high level of productivity after repeated use. Reduced form of nicotinamide adenine dinucleotide (NADH) recycling also occurred effectively for the enzymes immobilized on the fiber. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010