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Synthesis of hyperbranched poly(3‐methyl‐3‐hydroxymethyloxetane) and their application to separate basic proteins by adsorption coated column
Author(s) -
Shou Chongqi,
Song Nanjing,
Zhang Zhiliang
Publication year - 2010
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.31272
Subject(s) - capillary action , adsorption , lysozyme , cationic polymerization , branching (polymer chemistry) , materials science , chromatography , polymer chemistry , chemistry , chemical engineering , analytical chemistry (journal) , composite material , organic chemistry , biochemistry , engineering
A series of hyperbranched poly(3‐methyl‐3‐hydroxymethyloxetane)s with different degree of branching were synthesized using BF 3 ·OEt 2 as initiator and coated on the inner surface of the fused‐silica capillaries. In the pH range of 3–9, the coated capillaries reduced electro‐osmotic flow by about four times lower than the bare fused‐silica capillary. The coated capillaries also displayed good resistance to adsorption of cationic proteins, providing clean separations of a mixture of Lysozyme, Cytochrome c , and Ribonuclease A around pH 3–6 in phosphate buffer. The separation efficiency in terms of peak shape was excellent compared with bare fuse‐silica capillary. The separation efficiency of hyperbranched poly(3‐methyl‐3‐hydroxymethyloxetane) with degree of branching of 0.43‐coated capillary column for Lysozyme reached 10 6 plates/m with a resolution of 7.1, and the coated capillary column had good migration time reproducibility. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010