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In vitro release kinetics of bovine serum albumin from highly swellable dextran hydrogels
Author(s) -
İmren Dilek,
Gümüşderelioğlu Menemşe,
Güner Ali
Publication year - 2009
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.31042
Subject(s) - self healing hydrogels , dextran , epichlorohydrin , dextranase , bovine serum albumin , swelling , chemistry , polysaccharide , polymer chemistry , albumin , kinetics , diffusion , serum albumin , poloxamer , chemical engineering , materials science , chromatography , polymer , biochemistry , copolymer , organic chemistry , composite material , engineering , thermodynamics , physics , quantum mechanics
Hydrogels synthesized from a polysaccharide‐based polymer, dextran, in the presence of two crosslinking agents, N,N ′‐methylenebisacrylamide and epichlorohydrin, were evaluated for the oral colon‐specific delivery of polypeptide drugs. These novel dextran hydrogels had significantly greater swelling ratios than recently developed dextran hydrogels. A model protein, bovine serum albumin, was loaded into 50% (by weight) crosslinker‐containing dextran hydrogels in two ways: during the crosslinking reaction and by a soaking method. The loading capacity was varied between 22 and 25 mg/g of dry gel, depending on the loading procedure. In vitro release experiments were performed with a simulated gastrointestinal system in the presence and absence of dextranase. The diffusion exponents were calculated by means of a semiempirical power‐law equation for the release of protein from swellable hydrogel discs. Bovine serum albumin was mainly released by Fickian diffusion, and this indicated that its hydrodynamic diameter (7.7 nm) was smaller than the hydrogel mesh size (∼19 nm). The release of bovine serum albumin from both hydrogel types was substantially higher than expected, especially in the presence of dextranase, and this was attributed to the high swellability of the hydrogels. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010