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Role of PLLA plasma surface modification in the interaction with human marrow stromal cells
Author(s) -
Armentano Ilaria,
Ciapetti Gabriela,
Pennacchi Manuela,
Dottori Mariaserena,
Devescovi Valentina,
Granchi Donatella,
Baldini Nicola,
Olalde Beatriz,
Jurado Maria Jesus,
Alava Jose Inaki Marquinez,
Kenny José M.
Publication year - 2009
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.31008
Subject(s) - fluorescein isothiocyanate , protein adsorption , adhesion , materials science , stromal cell , adsorption , cell adhesion , biophysics , surface modification , plasma enhanced chemical vapor deposition , fluorescein , polymer , chemical engineering , bovine serum albumin , chemistry , fluorescence , nanotechnology , thin film , chromatography , composite material , organic chemistry , medicine , physics , pathology , quantum mechanics , engineering , biology
The effects of oxygen‐based radio frequency plasma enhanced chemical vapor deposition (rf PECVD) on the surface of poly( L ‐lactide) (PLLA) polymers and the influence thereof on protein adsorption and on bone–cell behavior have been studied. Thin films and porous scaffolds based on PLLA polymer were developed, and the role of surface modifications were investigated extensively. PECVD surface treatments were used to alter surface functionality and modulate protein adsorption on the PLLA polymer matrix. In particular, Bovine Serum Albumine fluorescein isothiocyanate (fitc‐BSA) conjugate adsorption on patterned surfaces of treated PLLA was analyzed by fluorescence microscopy. Human marrow stromal cells (MSCs) were cultured on scaffolds and cell adhesion and morphology were assessed using fluorescence microscopy. The results indicated that the PLLA surface became hydrophilic and its roughness increased with the treatment time and it had a dominant influence on the adsorption process of the protein. The outcome of the plasma treatment of various PLLA surfaces has been shown to be the up‐regulator of the cell‐adhesive proteins expression and consequently the improvement of cell adhesion and growth. Oxygen‐treated PLLA promoted higher adhesion and proliferation of the MSCs in comparison to the untreated samples. It can be concluded that following plasma treatment, PLLA samples show enhanced affinity for osteoprogenitor cells. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009