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Suppression of breast cancer cells in vitro by polyamidoamine‐dendrimer‐mediated 5‐fluorouracil chemotherapy combined with antisense micro‐RNA 21 gene therapy
Author(s) -
Mei Mei,
Ren Yu,
Zhou Xuan,
Yuan XuBo,
Li Fei,
Jiang LingHuo,
Kang ChunSheng,
Yao Zhi
Publication year - 2009
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.30868
Subject(s) - gene knockdown , rna , breast cancer , dendrimer , microrna , cancer research , fluorouracil , in vitro , cancer cell , genetic enhancement , antisense rna , apoptosis , chemistry , biology , microbiology and biotechnology , cancer , gene , biochemistry , genetics
A specific micro‐RNA (miRNA), micro‐RNA 21 (miR‐21), is strongly overexpressed in breast cancer cells. Antisense inhibition of miRNA function, an important tool for uncovering miRNA biology, which is often used to knockdown miRNA, can cause a notable inhibition of cell growth. In this study, 5‐fluorouracil (5‐FU) was conjugated to polyamidoamine dendrimers via direct encapsulation; this method was then combined with antisense micro‐RNA 21 (as‐miR‐21) strategies to evaluate the effects of the growth suppression of breast cancer cells. Our results show that as‐miR‐21 strategies significantly improved the chemosensitivity of free 5‐FU on breast cancer cells (MCF‐7). In addition, not only could as‐miR‐21 effectively increase the apoptotic cell numbers but it could also bring down the migration ability of MCF‐7 cells. Our results provide invaluable information for the future design of drug–polymer complexes for multimodal cancer treatments. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009