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Potentiometric studies on the interaction between superoxide dismutase and hyaluronic acid
Author(s) -
Fan Yaofeng,
Wang Yig,
Fan Yunge,
Ma Jianbiao
Publication year - 2009
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.30238
Subject(s) - potentiometric titration , titration , polyelectrolyte , chemistry , superoxide dismutase , ionic strength , titration curve , hyaluronic acid , turbidimetry , coacervate , nuclear chemistry , inorganic chemistry , chromatography , biochemistry , antioxidant , organic chemistry , polymer , ion , aqueous solution , biology , genetics
Abstract The formation process of soluble complexes and insoluble aggregates between superoxide dismutase (SOD) and hyaluronic acid (HA) was studied using quasielastic light scattering and turbidimetric titration. The electrostatic binding between them was investigated in detail through potentiometric titration and turbidimetric titration carried out from high to low pH. Turbidimetric titration was used to determine the specific pH values at which soluble complex formation was initiated (pH c ) and phase separation occurred (pH ϕ ). An increase of the ionic strength causes a decrease of pH c and pH ϕ . With the increase of HA concentrations, pH ϕ increases but pH c does not vary. The formed “salt bridges” between $-{\rm NH_3^+}$ (SOD) and COO − (HA) result in the formation of stable SOD‐HA complexes and even aggregates. The necessary condition of electrostatic binding was also given for protein‐acidic polyelectrolyte systems. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009