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Preparation of bovine serum albumin‐imprinted calcium polyacrylate/alginate hybrid microspheres via Ca 2+ crosslinking
Author(s) -
Zhao Kongyin,
Wei Junfu,
Cheng Guoxiang,
Yang Chunxia,
Chen Li
Publication year - 2009
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.29936
Subject(s) - sodium polyacrylate , calcium alginate , chemistry , bovine serum albumin , polymer , calcium , chromatography , polymer chemistry , chemical engineering , molecular imprinting , nuclear chemistry , selectivity , organic chemistry , raw material , engineering , catalysis
Macromolecularly imprinted calcium polyacrylate/alginate hybrid polymer microspheres with the imprinting of bovine serum albumin were prepared with sodium alginate and sodium polyacrylate by using CaCl 2 as a gelling agent in inverse‐phase suspension. Infrared spectrum and conductance titration demonstrated that hybrid components were produced in these microspheres. Rebinding isotherms of the microspheres proved a greater bovine serum albumin affinity for imprinted microspheres relative to nonimprinted ones. Selectivity tests indicated that calcium polyacrylate/alginate hybrid polymer microspheres exhibited good recognition properties for the template protein with the separation factor over 5.30 comparing to the competitive proteins with a similar M w . The influence of cross‐linker CaCl 2 concentration on the imprinting efficiency (IE) and rebinding capacity was researched. It was found that the cross‐linking density and imprinting efficiency of the hybrid hydrogel could be improved simultaneously. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009