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Magnetic polymer microspheres with azidocarbonyl groups: Synthesis, characterization and application in protein immobilization
Author(s) -
Guo Na,
Wu Daocheng,
Pan Xinghua,
Lu Maolin
Publication year - 2009
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.29755
Subject(s) - materials science , thermogravimetric analysis , superparamagnetism , polymer , fourier transform infrared spectroscopy , magnetic nanoparticles , magnetic force microscope , particle size , chemical engineering , microsphere , magnetometer , polymer chemistry , analytical chemistry (journal) , nanotechnology , magnetization , magnetic field , chemistry , composite material , nanoparticle , organic chemistry , physics , quantum mechanics , engineering
A novel magnetic polymer microsphere with amide groups and carboxyl groups was synthesized and reported here. The azidocarbonyl groups were derived from amide groups and linked to the proteins to investigate their immobilization capacity. The morphology, size, functional groups and magnetic properties of magnetic microspheres were characterized by optical microscopy, particle size analyzer, atom force microscopy, magnetic force microscopy, fourier transform infrared spectrometer, vibrating‐sample magnetometer and thermal gravimetric analysis. The results indicated that the magnetic polymer microspheres had a well spherical shape with the size ranging from 1 to 10 μm, highly reactive functional groups, superparamagnetism and strong magnetic responsibility with saturation magnetization of 18.443 emu/g and Fe 3 O 4 content around 21%. The immobilization capacity (η) was over 70%. The novel azidocarbonyl magnetic polymer microspheres showed potentials to be a good magnetic support and promising applications in bioseparation and biomedical fields. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009