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Immobilization cyclen copper (II) on merrifield resin: Efficient oxidative cleavage of plasmid DNA
Author(s) -
Li Kun,
Zhang Ji,
Zhang ZhongWei,
Xiang YongZhe,
Lin HongHui,
Yu XiaoQi
Publication year - 2008
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.29266
Subject(s) - cyclen , agarose gel electrophoresis , catalysis , agarose , cleavage (geology) , nuclear chemistry , copper , chemistry , scanning electron microscope , polymer chemistry , dna , combinatorial chemistry , materials science , stereochemistry , organic chemistry , biochemistry , fracture (geology) , composite material
Merrifield resin‐supported cyclen (MRC) was directly prepared by attaching 1, 4, 7, 10‐tetraazacyclododecane (cyclen) to Merrifield resin (MR). Subsequent coordination with Cu(II), Co(II), and Ni(II) gave immobilized cyclen complex MRC‐Cu, MRC‐Co, and MRC‐Ni as “solid artificial enzymes.” These complexes were characterized by elemental analysis, IR spectroscopy, ICP‐AES, and scanning electron microscopy (SEM). Furthermore, the DNA cleavage activities of these complexes were investigated by agarose gel electrophoresis. The results indicated that MRC‐Cu was superior to other solid artificial enzymes, and the cleavage process was carried out via oxidative pathway. Moreover, the solid catalyst MRC‐Cu was very stable and it could be reused at least four times without any loss of catalytic activity. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009