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Free radical scavenging activity of cellulase‐treated chitosan
Author(s) -
Feng Tao,
Gong Jian,
Du Yumin,
Huang Zili
Publication year - 2008
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.29115
Subject(s) - chemistry , chitosan , hydroxyl radical , cellulase , hydrolysis , scavenging , nuclear chemistry , superoxide , cellulose , radical , organic chemistry , polymer chemistry , antioxidant , enzyme
Partially N ‐acetylated chitosan was hydrolyzed by the cheap, commercially available, and efficient cellulase. The products, with different molecular weight, were comparatively investigated by GPC, FT‐IR, XRD, and NMR. The results show that the decrease of molecular weight led to transformation of crystal structure and increase of water‐solubility, but the chemical structures of residues were not modified. Superoxide anion radical and hydroxyl radical quenching assay were used for the evaluation of free radical scavenging activity of cellulase‐treated chitosan in vitro . Low molecular weight chitosan (LMWC3, M w 1.7 × 10 3 ) exhibited high scavenging activity against free radical. It scavenged 79.3% superoxide radical at 0.1 mg mL −1 . At 2.0 mg mL −1 , scavenging percentage of initial chitiosan, LMWC1 ( M w 27.3 × 10 3 ), LMWC2 ( M w 5.9 × 10 3 ), and LMWC3 ( M w 1.7 × 10 3 ) against hydroxyl radical was 14.3%, 33.1%, 47.4%, and 65.9%, respectively. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009