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N ‐acetyl‐ D ‐galactosamine‐specific lectin isolation from soyflour with poly(HPMA‐GMA) beads
Author(s) -
Perçin Işık,
Yavuz Handan,
Aksöz Erol,
Denizli Adil
Publication year - 2008
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.29054
Subject(s) - lectin , soybean agglutinin , glycidyl methacrylate , adsorption , chemistry , chromatography , galactosamine , nuclear chemistry , galactose , biochemistry , organic chemistry , polymer , agglutinin , copolymer
Soybean lectin was purified from seeds of Glycine max L.Merrill SA88. Poly(hydroxypropyl methacrylate‐glycidyl methacrylate) [poly(HPMA‐GMA)] beads were used as an affinity matrix and N ‐acetyl‐ D ‐galactosamine (GalNAc) was used as an affinity ligand. Soybean lectin adsorption with GalNAc attached poly(HPMA‐GMA) beads from soybean lectin solution (in phosphate buffered saline) was 5.0 mg/g. Maximum adsorption capacity for soybean lectin from the soy flour extract was 26.0 mg/g. Elution of soybean lectin from adsorbent was accomplished by 0.5 M galactose solution. Purity of soybean lectin was determined by SDS‐PAGE. It was observed that soybean lectin could be repeatedly adsorbed and desorbed with GalNAc‐attached poly(HPMA‐GMA) beads. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009