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Polysaccharide‐based artificial extracellular matrix: Preparation and characterization of three‐dimensional, macroporous chitosan, and heparin composite scaffold
Author(s) -
Yu ShuHuei,
Wu YuBey,
Mi FwuLong,
Shyu ShinShing
Publication year - 2008
Publication title -
journal of applied polymer science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.575
H-Index - 166
eISSN - 1097-4628
pISSN - 0021-8995
DOI - 10.1002/app.28494
Subject(s) - chitosan , glutaraldehyde , carbodiimide , biocompatibility , composite number , tissue engineering , biomaterial , polymer , materials science , scaffold , polymer chemistry , chemical engineering , chemistry , nuclear chemistry , biomedical engineering , composite material , nanotechnology , chromatography , biochemistry , medicine , engineering , metallurgy
Scaffold‐guided tissue engineering based on synthetic and natural occurring polymers has gained many interests in recent year. In this study, the development of a chitosan‐heparin artificial extracellular matrix (AECM) is reported. Three‐dimensional, macroporous composite AECMs composed of heparin (Hep) and chitosan (Chito) were prepared by an interpolyelectrolyte complex/lyophilization method. The Chito‐Hep composite AECMs were, respectively, crosslinked with glutaraldehyde, as well as cocrosslinked with N,N ‐(3‐dimethylaminopropyl)‐ N ′‐ethyl carbodiimide (EDC/NHS) and N ‐hydroxysuccinimide (NHS). The crosslinking reactions were examined by FT‐IR analysis. In physiological buffer solution (PBS), the EDC/NHS‐crosslinked Chito‐Hep composite AECM showed a relative lower water retention ratio than its glutaraldehyde‐crosslinked counterparts. The EDC/NHS‐crosslinked Chito‐Hep composite AECMs showed excellent biocompatibility, according to the results of the in vitro cytotoxic test. This result suggested that the EDC/NHS‐crosslinked Chito‐Hep composite AECMs might be a potential biomaterial for scaffold‐guided tissue engineering applications. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008